Coding

Part:BBa_K5181003:Design

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-13)


CPR_truncated: Sandalwood CPR without transmembrane domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1252
    Illegal PstI site found at 1531
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While truncating the sequence, 147 nucleotides from position 3 to position 150 were removed from N-terminal.

The Amino acid sequence is: MGEKSKELRPVVALKAAPIEAEEDDGEVDSGKTKVTVFFGTQTGTAEGFAKALAEEIKARYEKAVVKVVDLDDYAADDDQYGEKLKNETLTFFMVATYGDGEP TDNAARFYKWFTEEKEREAWLQQLTYGIFGLGNRQYEHFNKIAKVLDEQLTEQGAKRLIQVGLGDDDQCIEDDFSAWRELLWPELDQLLRGDDGANSVSTPYT AAVPEYRVVIHDPTITSSEDKSLATANGAALFDIHHPCRVKVAVQRELHKADSDRSCIHLEFDISGTGLMYETGDHVGVYAENCVETVEEAGKLLGQPLDLLF SVHTDKDDGTSLESSLPPPFPGPCTLRTALFQYADLLNPPRKAALVALAAQAVEPSEADRLKFLSTPQGKDEYAKWVVGSQRSLLEVMAEFPSIKVPLGVFFA AVAPRLQPRYYSISSSPRFSSDRVHVTCALVYGPSPTGRIHRGVCSTWMKNAVPLEESRECSWAPIFIRTSNFKLPANPSTPVIMVGPGTGLAPFRGFLQERM ALLEGSAQLGPALLFFGCRNRRMDFIYEDELNNFVEQGVISELIVAFSRDGPTKEYVQHKMMDKAAYIWSLISQGAYLYVCGDAKGMARDVHRTLHTLVQQQE SVDSSKAESIVKKLQMDGRYLRDVWHHHHHH

The protein has a 6X His tag on its C-termini and can be purified by a Ni-NTA column.

Source

The part is derived from BBa_K5181001, which was obtained from NCBI(KC842187.1) from Chavez et al. Biosynthesis of Sandalwood Oil: Santalum album CYP76F Cytochromes P450 Produce Santalols and Bergamotol (2013)

References